Dose Effects of Recombinant Adenovirus Immunization in Rodents

Production of Recombinant Adenovirus Containing Human Interlukin-4 Gene

Objective(s) Recombinant adenoviruses are currently used for a variety of purposes, including in vitro gene transfer, in vivo vaccination, and gene therapy. Ability to infect many cell types, high efficiency in gene transfer, entering both dividing and non dividing cells, and growing to high titers make this virus a good choice for using in various experiments. In the present experiment, a reco...

Toxic effects of high-dose cyanate administration in rodents.

Cyanate inhibits hemoglobin synthesis in vitro. In vivo studies were performed to determine the effect of the drug on erythropoiesis. Adult female rats received daily intraperitoneal injections of 400 /.Lmoles of cyanate, leading to over 3 CNO/Hb by 3 wk. Controls received sodium chloride. DPG was measured in a small number of animals and declined from 5.5 to 4.1 mmoles/liter (p <0.02). At 4 wk...

Delivery of Recombinant Adenovirus

Background Gene transfer can potentially alter vessel wall biology and intervene in the pathogenesis of human disease. Although several methods for vector delivery have been described, systematic comparisons of these methods are unavailable. Therefore, this study compared three catheter-based strategies and a surgical technique to assess efficient and selective gene transfer to the vascular wal...

Induction of Tumor-Protective Immunity and Adenovirus Encoding a Lymphoma Idiotype: Immunization with a Recombinant

Rapid functional exhaustion and deletion of CTL following immunization with recombinant adenovirus.

Replication-deficient adenoviruses (recombinant adenovirus (rec-AdV)) expressing different transgenes are widely used vectors for gene therapy and vaccination. In this study, we describe the tolerization of transgene-specific CTL following administration of beta-galactosidase (beta gal)-recombinant adenovirus (Ad-LacZ). Using MHC class I tetramers to track beta gal-specific CTL, we found that a...